Germination Kinetics and Chlorophyll Fluorescence Imaging Allow for Early Detection of Alkalinity Stress in Rhododendron Species

刊物

Horticulturae 2022

刊号

ISSN 2311-7524

摘要

Rhododendron species are typical calcifuges that do not grow well in calcareous soils characterized by  alkaline pH and high concentrations of Ca²⁺. In this study,we investigated the effects of three pH levels and a Ca²⁺ treatment on the in vitro germination and seedling growth of three Rhododendron species:R.chihsinianum,R.fortunei, and R.vernicosum. Alkaline pH had no significant effect on germination parameters (gMAX, mean germination time and germination uniformity) but significantly increased abnormal leaf development (AL) and mortality in all three species. Adding extra Ca²⁺ reduced the mean germination time  for R.vernicosum. The negative influence of alkaline pH on seedlings was already visible on the second day of treatment as measured by chlorophyll fluorescence imaging parameters (Fv/Fm and ΦPSII) on R. fortunei . Extra Ca²⁺ alleviated the negative effect of alkaline pH and increased Fv/Fm 41 days after seed germination in R.fortunei  and R.chihsinianum and reduced mortality for all three species. In conclusion, alkaline pH mainly influenced seedling development and growth but not the germination process itself. Chlorophyll fluorescence imaging can be an efficient way to perform high-throughput in vitro screening of Rhododendron seedlings for alkalinity tolerance.