Educational Background

2015-2019 Ph.D. Huazhong Agricultural University Wuhan China.

2013-2015 M.Phil. Quaid-i-Azam University Islamabad Pakistan.

2011-2013 M.Sc. Quaid-i-Azam University Islamabad Pakistan.

2008-2010 B.Sc. University of Sindh Jamshoro  Sindh Pakistan. 

Work Experience

2021- to date Associate Researcher Lushan Botanical Garden, Chinese Academy of Sciences, China.

2019-2021 Postdoctoral Researcher South China Agricultural University, Guangzhou,China.

Research Interests

To study the physiological response and molecular mechanism of plants under different abiotic stress conditions.  

Scientific research projects undertaken:

China Postdoctoral Science Foundation,2020M682721,Functional Characterization and Molecular Mechanism of AtSAL1 against Endoplasmic reticulum stress tolerance in Arabidopsis thaliana. (Host)

Honours and awards

1. Selected as an excellent Alumni(PhD 2019)at Huazhong Agricultural University, Wuhan, Hubei,China.

2. Secured second position in poster presentation in“2nd SINO-PAK International Conference on Innovations in Cotton Breeding & Biotechnology”,November 26–27,2018 at Muhammad Nawaz Shareef University of Agriculture Multan,Pakistan.

3. Got Chinese Government Scholarship to pursue a PhD for the years;2015–2019.

4. Indigenous Ph.D., Fellowships, Higher Education Commission (HEC), Govt. of Pakistan (For MPhil leading to PhD 2013 to 2017),not availed.

5. Prime Minister’s Laptop award during MPhil.

6. admission of MPhil degree in 2013 in Quaid-i-Azam University,Islamabad,Pakistan. 

Publications

1. Qurban Ali,Chenjie Yu,Amjad Hussain,Mohsin Ali, Sunny Ahmar, Muhammad Aamir Sohail,Muhammad Riaz,Dyaaaldin Abdalmegeed, Hakim Manghwar*,Lei Zhou1.Genome engineering technology for durable disease resistance;recent progress and future outlook in sustainable agriculture.Frontiers in Plant Science, 13:860281.doi:10.3389/fpls.2022.860281.IF:5.753

2. Qurban Ali#*,Hongxia Zheng#,Muhammad Junaid Rao,Mohsin Ali,Muhammad Hamza Saleem,Yasser Nehela,Muhammad Aamir Sohail, Agha Mushtaque Ahmed, Kashif Ali Kubar,Shafaqat Ali,Hakim Manghwar*,Lei Zhou.Advances,limitations, and prospects of biosensing technology for detecting phytopathogenic bacteria. Chemosphere,296(2022)133773. https://doi.org/10.1016/j.chemosphere.2022.133773.IF:7.086

3. Hakim Manghwar*,Amjad Husssain,Qurban Ali,and Fen Liu*.Brassinosteroids (BRs) Role in Plant Development and Coping with Different Stresses.International Journal of Molecular Sciences, 2022,23,1012.https://doi.org/10.3390/ijms23031012.IF:5.923

4. Hakim Manghwar and Jianming Li*.Endoplasmic Reticulum Stress and Unfolded Protein Response Signaling in Plants. International Journal of Molecular Sciences, 2022,23,828. https://doi.org/10.3390/ijms23020828.IF:5.923

5. Hakim Manghwar,Amjad Hussain,Qurban Ali, Muhammad Hamzah Saleem,Muyassar H.Abualreesh,Aishah Alatawi,Shafaqat Ali* and Muhammad Farooq Hussain Munis*(2021)Disease severity, resistance analysis and expression profiling of pathogenesis-related protein genes after the inoculation of Fusarium equiseti in wheat.Agronomy 2021,11(11), 2124; https://doi.org/10.3390/agronomy11112124

6. Hakim Manghwar* and Amjad Hussain(2021).Mechanism of tobacco osmotin gene in plant responses to biotic and abiotic stress tolerance;a brief history.BIOCELL ID:17316.

7. Amjad Hussain;Xiao Ding;Muna Alariqi;Hakim Manghwar;Fengjiao Hui; Yapei Li; Junqi Cheng;Chenglin Wu; Jinlin Cao; Shuangxia Jin*.Herbicide resistance: another hot agronomic trait for genome editing. Plants 2021,10,621. https://doi.org/10.3390/plants10040621.

8. Debin Zhan†;Amjad Hussain†;Hakim Manghwar;Kabin Xie;Shengsong Xie;Shuhong Zhao;Robert M.Larkin;Ping Qin;Shunagxia Jin; Fang Ding*.Genome editing with the CRISPR-Cas system: an art, ethics and global regulatory perspective.Plant Biotechnology Journal,(2020)18,pp.1651–1669.

9. Aamir Hamid Khan, Ling Min,Yizan Ma,Yuanlong Wu,Yuanhao Ding,Yanlong Li,Sai Xie,Abid Ullah,Muhammad Shaban,Hakim Manghwar,Muhammad Shahid, Yunlong Zhao,Chaozhi Wang and Xianlong Zhang*.High day and night temperatures distinctively disrupt fatty acid and jasmonic acid metabolism, inducing male sterility in cotton. Journal of Experimental Botany,Vol.71,No.19 pp. 6128–6141,2020 doi:10.1093/jxb/eraa319.

10. Hakim Manghwar,Bo Li,Xiao Ding,Amjad Hussain,Keith Lindsey,Xianlong Zhang§,Shuangxia Jin§.CRISPR/Cas Systems in Genome Editing:Methodologies and Tools for sgRNA Design, Off-target Evaluation and Strategies to Mitigate Off-target Effects. Advanced Science,2020,1902312,DOI:10.1002/advs.201902312.

11. Hakim Manghwar,Keith Lindsey,Xianlong Zhang§,Shuangxia Jin§.CRISPR/Cas System: Recent Advances and Future Prospects for Genome Editing. Trends in Plant Science. Volume 24, Issue 12, December 2019, Pages 1102-1125. https://doi.org/10.1016/j.tplants.2019.09.006.

12. Lei Qin;Jianying Li;Qiongqiong Wang;Zhongping Xu;Lin Sun;Muna Alariqi;Hakim Manghwar;Guangying Wang;Bo Li;Xiao Ding;Hangping Rui;Huimei Huang; Tianliang Lu;Keith Lindsey;Henry Daniell;Xianlong Zhang;Shuangxia Jin*.High Efficient and Precise Base Editing of C•G to T•A in the Allotetraploid Cotton (Gossypium hirsutum) Genome using a Modified CRISPR/Cas9 System.Plant Biotechnology Journal,(2020)18, pp.45–56.

13. Amjad Hussain,Amna,Muhammad Aqeel Kamran,Muhammad Tariq Javed,Kashif Hayat,Muhammad Asad, Farooq,Naeem Ali,Musrat Ali,Hakim Manghwar,Farooq Jan,Hassan Javed Chaudhary*.Individual and combinatorial application of Kocuria rhizophila and citric acid on phytoextraction of multi-metal contaminated soils by Glycine max L.Environmental and Experimental Botany,159(2019)23–33.

14. Li, Jianying†;Manghwar,Hakim†(First co-author);Sun,Lin†; Wang, Pengcheng;Wang,Guanying;Sheng,Hanyan;Zhang,Jie;Liu, Hao; Qin,Lei; Rui,Hangping; Li,Bo;Lindsey,Keith;Daniell,Henry;Jin, Shuangxia*; Zhang, Xianlong. Whole genome sequencing reveals rare off‐target mutations and considerable inherent genetic or/and somaclonal variations in CRISPR/Cas9‐edited cotton plants.Plant Biotechnology Journal(2018),pp.1–11, https://doi.org/10.1111/pbi.13020.

15. Abid Ullah,Hakim Manghwar,Muhammad Shaban,Aamir Hamid Khan,Adnan Akbar, Usman Ali,Ehsan Ali, Shah Fahad(2018).Phytohormones enhanced drought tolerance in plants:a coping strategy.Environmental Science and Pollution Research.https://doi.org/10.1007/s11356-018-3364-5.

16. Abid Ullah,Adnan Akbar,Qingqing Luo,Aamir Hamid Khan,Hakim Manghwar,Muhammad Shaban,Xiyan Yang*(2018).Microbiome Diversity in Cotton Rhizosphere Under Normal and Drought Conditions.Microbial Ecology.https://doi.org/10.1007/s00248-018-1260-7.

17. Lizhen ZHU,Jianying LI,Zhongping XU,Hakim MANGHWAR,Sijia LIANG, uli LI,Muna ALARIQI, Shuangxia JIN*,Xianlong ZHANG (2018).Identification and selection of resistance to Bemisia tabaci among 550 cotton genotypes in the field and greenhouse experiments. Frontiers of Agricultural Science and Engineering https://doi.org/10.15302/J-FASE-2018223.

18. Abid Ullah,Hafsa Mushtaq,Usman Ali,Hakim,Ehsan Ali,Samavia Mubeen.Screening, isolation,biochemical and plant growth promoting characterization of endophytic bacteria.Microbiology: Current Research,2018 Volume 2 Issue 2.

19. Hakim Manghwar,Amjad Hussain,Abid Ullah,Summia Gul,Muhammad Shaban, Aamir Hamid Khan, Musrat Ali, Syed Gul Abbas Shah Sani, Hassan Javed Chaudhary, Muhammad Farooq Hussain Munis* (2018).Expression analysis of defense related genes in wheat and maize against Bipolaris sorokiniana. Physiological and Molecular Plant Pathology 103:36–46.

20. Muhammad Shaban,Yuhuan Miao,Abid Ullah,Anam Qadir Khan,Hakim Menghwar,Aamir Hamid Khan,Muhammad Mahmood Ahmed, Muhammad Adnan Tabassum, Longfu Zhu*.(2018).Physiological and molecular mechanism of defense in cotton against Verticillium dahliae.Plant Physiology and Biochemistry 125: 193–204.

21. Hakim,Abid Ullah,Amjad Hussain,Muhammad Shaban,Aamir Hamid Khan, Muna Alariqi,Summia Gul, Zhang Jun,Sun Lin, Jianying Li, Shuangxia Jin*,Muhammad Farooq Hussain Munis* (2018).Osmotin:A plant defense tool against biotic and abiotic stresses.Plant Physiology and Biochemistry 123:149–159.

22. M.Farooq Hussain Munis*,Shicheng Xu,Hakim,Samia Naz, Hassan Javed Chaudhary,Sajid Masood,Abu Bakr Umer Farooq (2018). Diagnosis of Fusarium graminearum in Soil and Plant Samples of Wheat by Real-Time PCR.Romanian Biotechnological Letters,Vol.23,No. 5,2018, DOI 10.26327/RBL2018.223.

23. Abid Ullah,Heng Sun,Hakim,Xiyan Yang* and Xianlong Zhang (2017).A novel cotton WRKY gene, GhWRKY6-like,improves salt tolerance by activating the ABA signaling pathway and scavenging of reactive oxygen species.Physiologia Plantarum.doi:10.1111/ppl.12651.

24. Xin-Qi Cheng,Xue-Feng Zhu,Wen-Gang Tian,Wen-Han Cheng,Hakim,Jie Sun,Shuang-Xia Jin,Hua-Guo Zhu (2017).Genome-wide identifcation and expression analysis of polyamine oxidase genes in upland cotton(Gossypium hirsutum L.). Plant Cell, Tissue and Organ Culture(PCTOC). 129,237–249. DOI 10.1007/s11240-017-1172-0.

25. A. Ullaha,H.Mushtaq,Sh.Fahad,Hakim,A.Shah,and H. J. Chaudhary*.Plant Growth Promoting Potential of Bacterial Endophytes in Novel Association with Olea ferruginea and Withania coagulans (2017).ISSN 0026-2617,Microbiology,2017, Vol. 86,No.1,pp.119–127.

26. Noor Muhammad,Hakim,Umar Masood Quraishi, Hassan Javed Chaudhary, Muhammad Farooq Hussain Munis*(2016).IAA(indole-3-acetic acid)induces biochemical and physiological changes in wheat under drought stress. PHILIPP AGRIC SCIENTIST Vol.99 No.1,19–24.

27. Hakim,S.Naz,S.Gul,H.J.Chaudhary and M.F.H.Munis*(2015). FIRST REPORT OF RHIZOPUS ORYZAE CAUSING FRUIT ROT OF CITRUS MEDICA L.IN PAKISTAN.Journal of Plant Pathology,97(1),209–220.

28. Hakim,S.Naz,F.Liaquat,S.Gul,H.J.Chaudhary and M.F.H.Munis* (2015).PRESENCE OF BACILLUS PUMILUS CAUSING FRUIT ROT OF FICUS LACOR IN PAKISTAN.Journal of Plant Pathology,97(3),541–551.